| 1. | The fifth part summaried how to cultivate human skin fibroblasts in vitro . 1
为了开展研究，第5章进行了人皮肤成纤维细胞的培养。 |
| 2. | Monolayer human skin fibroblasts were used again to study the photobiomodulation of marginal irradiation of high intensity laser beam in the fourth part
本文的第四章探讨了高强度nd : yag和ktp倍频激光束边缘对单层人成纤维细胞的光生物调节作用。 |
| 3. | The surface biocompatibility was also greatly improved after modification ; human skin fibroblasts adhered quickly and grew well on the modified silicone surface
根据细胞吸附、生长实验可知，烯丙胺等离子体修饰后，表面的生物相容性有很大的改善。 |
| 4. | Effects of low intensity laser irradiation and led irradiation on the fibroblasts . the six parts of this thesis explored the effects of light emission diode ( led ) irradiation on the proliferation of human skin fibroblasts
低强度激光照射和led照射对成纤维细胞的影响发光二极管( led )照射能代替低强度激光照射在治疗伤口愈合中发挥作用。 |
| 5. | How to cultivate human skin fibroblasts in vitro was also summaried in this thesis . in the second part , with the help of mtt assay , monolayer human skin fibroblasts were used as models to study the damage effects of nd : yag and ktp laser irradiation
这两种效应都会影响术后创口愈合过程；然后，介绍成纤维细胞体外培养在创伤愈合研究中的应用；最后，简述人皮肤成纤维细胞的培养方法。 |
| 6. | 2 . effects of ketamine in combination with he - ne laser irradiation on proliferation of human skin fibroblasts low intensity laser ( lil ) is being used clinically to enhance wound healing . unfortunately , the animal experimental results were controversial
氯胺酮对he补e激光照射促人正常皮肤成纤维细胞增殖作用的影响低强度激光照射治疗伤口愈合疗效显著，但是，动物实验结果的不一致一直是阻碍其发展的严重障碍。 |
| 7. | In fact , the intensity of high power laser beem decreases rapidly when laser is transmissing in bio - tissue and the intensity of the edge of laser beem is so low that photobiomodulation , which might effect the healing process after laser surgery , but not thermal effect might work . in this thesis , monolayer human skin fibroblasts was used as models to study the damage effect of high intensity laser irradiation and photobiomodulation of marginal irradiation of high intensity laser beam
高强度激光在生物组织中迅速衰减，激光束边缘强度低到无法损伤细胞，这部分激光对已损伤组织边缘存活细胞的光生物调节作用(例如，对细胞增殖的促进或抑制效应，对免疫细胞功能的调节)是决定高强度激光手术后生物修复状况的主要因素之一。 |
| 8. | In the seventh part , we used in vitro human skin fibroblasts as models to investigate whether ketamine affected laser - treated wound healing . the experiments investigated the effects of ketamine in combination with 468 . 7mj / cm2 he - ne laser irradiation on proliferation of in vitro human skin fibroblasts . cell proliferation was measured using the 3 - ( 4 , 5 - dimethylthiazol - 2 - yl ) - 2 , 5 - diphenyltetrazolium bromide ( mtt ) assay
用四甲基偶氮呻欢the3 （ 4 ， 5 dimethylthiazol 2 yi ） 2 ， 5 diphenyltetrazoliumbronnde （ mtt ） assay比色分析法测定了氯胺酮对hene激光照射促人正常皮肤成纤维细胞增殖作用的影响。 |
